I design and execute multicolor flow cytometry panels to profile lymphocyte and myeloid populations across tissues in WT and LRRK2 mouse models of Parkinson's disease, quantifying immune cell frequencies, activation states, and proliferative responses to PBS, PFF, and compound X1 treatments.
Panel 1 quantifies CD4+ T cells, CD8+ T cells, B cells, Tregs, and Ki67+ proliferating subsets across WT and LRRK2 genotypes treated with PBS, PFF, or compound X1. Bar charts show group means with SEM error bars and individual animal data points overlaid.
Arcsinh-transformed fluorescence histograms for Panel 3 myeloid markers — Ki67, CD11c, CD11b, F4/80, Ly6G, Ly6C — with all specimens overlaid and the unstained control shown as a dashed black line. These distributions reveal marker expression levels and the separation between positive and negative populations.
Forward scatter (FSC-A) vs side scatter (SSC-A) dot plots are the first step in the gating hierarchy, used to identify the main cell population and exclude debris and doublets before downstream immune phenotyping.