Full Technical Skills

Cell Culture & Disease Models

iPSC Platform

iPSC expansion, maintenance, QC (morphology, pluripotency markers, mycoplasma), banking, and differentiation across dopaminergic neurons, cortical/excitatory neurons, astrocytes, microglia-like cells, and endodermal lineages (hepatic, lung/epithelial, gut, beta islet). Neuronal maturation, co-culture paradigms (neuron–glia, neuron–microglia, immune–neural), 2D and 3D culture systems, organoid-like models, and scale-up for screening.

Mammalian Expression Systems

CHO and HEK293 expression systems for recombinant protein production. Pool-to-clone workflows, screening funnel design, stable line generation, expression benchmarking, and single-use bioreactor operation (5 L scale). Suspension and adherent culture with long-term stability.

Cell Lines

20+ cell lines including HEK293/293T, HeLa, U2OS, U87, U251, SH-SY5Y, A549, Calu-3, Caco-2, HMC3, THP-1, LN229, HepG2, Vero E6, COS-7, CHO, Hap1, and RAW264.7. Primary rodent neurons, primary human astrocytes, primary glia/oligodendrocytes, and PBMCs.

Biosafety

BSL-2 and BSL-3 laboratory experience, aseptic technique, sterility controls, environmental monitoring, and contamination prevention.

Genetic Engineering & Molecular Biology

Gene Perturbation

CRISPR/Cas9 knockout and knock-in, siRNA/shRNA-mediated knockdown, ASO/SSO-mediated silencing and modulation, lentiviral/adenoviral/AAV transduction, lipid-based transfection, and nucleofection/electroporation.

Molecular Cloning

Restriction cloning, Gibson assembly, Golden Gate cloning, primer design, PCR, bacterial transformation, colony screening, plasmid preparation (MiniPrep/MaxiPrep), Sanger sequencing, endotoxin-free DNA preparation, and construct design for membrane proteins and reporters.

Nucleic Acid & Protein Analysis

DNA/RNA extraction and QC, RT-qPCR/ddPCR, RNA-seq sample preparation and coordination, cDNA synthesis, Western blot (including Simple Western/Wes), SDS-PAGE, ELISA/MSD/Luminex multiplex, biochemical fractionation, immunoprecipitation, and His-tagged protein purification.

Cellular & Functional Assays

Imaging-Based Assays

Immunocytochemistry/immunofluorescence, live-cell imaging, cell painting (LysoTracker, Hoechst, MitoTracker, Cell Mask), high-content screening (Opera Phenix, Incucyte), calcium imaging, MEA-based electrophysiology, and multiplexed staining.

Flow Cytometry

Multicolor immunophenotyping, intracellular signaling readouts, cell-surface binding assays, gating strategy, compensation controls, activation-state profiling, and FlowJo analysis.

Plate-Based & Functional Readouts

CellTiter-Glo viability, FLIPR calcium flux and membrane potential (BMG FLUOstar Omega), AlphaLISA, TR-FRET, HTRF, BRET, luminescence/fluorescence plate-reader assays, dose-response and time-course profiling, and 96/384-well miniaturization.

Immune & Primary Cell Assays

PBMC isolation, culture/activation, and cytokine stimulation. Immune-target co-culture assays. T-cell functional assays. Primary human immune cell workflows. Cytokine quantification (ELISA/MSD/Luminex). Whole blood and hematopoietic cell matrices.

Extracellular Vesicle Biology

EV/exosome isolation from conditioned media, CD63/CD9 transfer assays, EV characterization by confocal microscopy, immunoblotting, and TEM. Exosome-mediated propagation assays.

Microscopy & Imaging

Confocal microscopy (Leica), Zeiss Airyscan super-resolution, Abberior STED microscopy, SIM, TIRF (Nikon), transmission electron microscopy (Tecnai), correlative light-electron microscopy (CLEM), negative-stain EM, live-cell imaging, widefield microscopy, high-content imaging (Opera Phenix, Incucyte), immunohistochemistry (IHC), cryosectioning, FFPE sectioning, RNAscope/FISH, and 3D z-stack volumetric analysis.

Data Science, Automation & Software

Programming & Analysis

Python (NumPy, pandas, scikit-learn, Jupyter), R, MATLAB, GraphPad Prism, FlowJo, JMP, ImageJ/Fiji, Imaris, PyMOL, Adobe Illustrator & Photoshop, SnapGene, and SoftMax Pro.

Automation

BRAVO liquid handling, Tecan Fluent, Hamilton, VIAFLO, Assist Plus, KingFisher sample prep automation. 96- and 384-well plate-based workflow standardization. Automation-ready assay design for high-throughput execution.

Computational Image Analysis

AutoMorphoTrack (custom Python package), adaptive segmentation, morphology classification, frame-by-frame tracking, colocalization analysis, feature extraction, batch QC, automated visualization, and machine learning methods for phenotypic fingerprinting.

Translational & Industry Experience

Biomarker Development

Target engagement (TE) and pharmacodynamic (PD) biomarker panel design. Imaging biomarkers, protein biomarkers (Western/ELISA/MSD), nucleic acid readouts (RT-qPCR, ddPCR, RNA-seq), and mass spectrometry–guided discovery. Biomarker hypothesis development, prioritization, and biological justification.

Documentation & Quality

ELN/LIMS discipline (LabArchives, Benchling), SOP authorship and review, technical reports, deviation tracking, OOS/OOT investigation awareness, GMP/CMC-quality mindset, and audit-ready recordkeeping.

Disease Areas

Parkinson's disease, Alzheimer's disease, frontotemporal dementia, multiple system atrophy, Duchenne muscular dystrophy, developmental epileptic encephalopathies, SARS-CoV-2 / infectious disease, and oncology (glioblastoma, cancer cell models, Parkin-mediated tumor biology).

In Vivo Experience

Rodent handling (mouse/rat), breeding, dosing/administration (i.p., i.v.), tissue collection, immune tissue processing, behavioral testing, and cross-model translation (human iPSC ↔ rodent systems).